Topoisomerase II alpha antibody

Catalog No. ABIN7120015

This Rabbit Polyclonal antibody specifically detects Topoisomerase II alpha in WB, IHC, ELISA, IF and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for Topoisomerase II alpha antibody (ABIN7120015)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-Topoisomerase II alpha Antibody

Purpose: TOP2A antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: topoisomerase(DNA) II alpha 170kDa

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background:

Synonyms: DNA topoisomerase 2-alpha|DNA topoisomerase II, alpha isozyme|TOP2A|TOP2

Background: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation(By similarity).

Molecular Weight: 174 kDa

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases